There are obvious differences between cancerous cells and normal cells in morphology, chemical properties and mechanical properties. The detection of cytochemical and mechanical properties of tumor tissues can provide multi-dimensional information for the pathological process of cells and human tissues.
This article has been reviewed according to Science X's editorial process and policies . Editors have highlighted the following attributes while ensuring the content's credibility: (a) Schematic configuration. (b) Instrument configuration. Credit: Lirong Qiu, Yunhao Su, Ke-Mi Xu, Han Cui, Dezhi Zheng, Yuanmin Zhu, Lin Li, Fang Li, Weiqian Zhao Among the existing detection methods for the morphology, mechanics and chemical properties of tissue and cells, confocal Raman spectroscopy can detect the chemical properties of micro-regions of samples without contact and label, and confocal Brillouin spectroscopy can detect the mechanical properties of micro-regions of samples without contact and nondestructive. Combining confocal Raman spectroscopy with Brillouin spectroscopy, simultaneously and in-situ, to detect the three-dimensional morphology, chemical properties and mechanical properties of micro-regions of tissues and even subcellular structures is expected to provide a new means for the detection of multi-dimensional pathological information of tissue and cells. The existing confocal Raman/Brillouin spectroscopic microscopic imaging technology has a lack of high precision real-time focusing ability, so that the size of the spot focused on the sample changes with the fluctuation of the sample in the scanning process, thus restricting the realization of the theoretical spatial resolution of confocal spectrum microscopy system. Secondly, due to the weak Raman and Brillouin scattering spectra and long integration time, confocal spectrum microscope is easily affected by system drift and leads to defocus, thereby affecting the spatial resolution and imaging quality. In addition, when it was used for biological tissue slice sample imaging, the fluorescence signal generated by vertical incidence reduced the signal-to-noise ratio of the sample's Raman spectrum. Consequently, this affects the accuracy of Raman spectrum and Brillouin spectrum detection and reduces the…